Construction of cDNA bank from biopsy specimens for multiple gene analysis of cancer.

The mRNAexpression study on small amount of Theileria annulata lymph node biopsy sample using SMART-cDNA technology

BACKGROUND: A major issue in many gene expressionstudies utilizing small amount of biological materials is thelimited quantity of RNApurified from clinical samples, which isoften used for RT-PCR or standard Northern blot analysis.OBJECTIVES: The SMART cDNA synthesis method and subsequentSMART-cDNA-PCR technique was used to analyse 3genes in macroschizonts of Theileria annulata in small lymphnod...

The Prevalence of P53 Mutations in Laryngeal Cancer in Kerman

Background &Aims: Laryngeal cancer is the second common cancer of respiratory tract, following the lung cancer. Carcinogenesis is a complex multistage process; molecular genetics has provided the evidence that activation of proto-oncogene and loss or inactivation of tumor suppressor genes (TSG) are involved in a large number of malignancies. One of the earliest significant tumor suppressor gene...

بررسی بیان ژن اینترلوکین-11 (IL-11) در بیماری ‌های خوش خیم و بدخیم مخاط معده

Background and purpose: Gastric cancer has been considered as the second lethal cancer throughout the world and the incidence of this cancer in Ïran is 25 in 10000 people per year. Ïnterleukin-11 (ÏL-11), a member of ÏL-6 family, may play some role in developing gastric cancer via activation of the JÂK/STÂT signaling pathway. Ïn this study, the level of ÏL-11 gene expression was determined in g...

PCR and RFLP Analysis for Identification and Typing of Helicobacter pylori Strains from Gastric Biopsy Specimens

Prevalence of Nucleotide Alterations of EGFR Gene in Patients with Esophageal Squamous Cell Carcinoma in Kerman

Background & Aims: Esophageal Cancer is the sixth fatal cancer in the world. Squamous and adenocarcinoma account for 95% of esophageal cancer. The expression of EGFR has a role in the pathophysiology of epidermal-based malignancies such as esophageal cancer. EGFR is also an important criterion in the evaluation of disease staging and prognosis. The aim of this study was to survey the prevalence...